lamp to detect fusarium graminearum contamination

(PDF) An Optimized Protocol for DNA Extraction from
2011.12.1 Specific PCR primer pair Tox5-1/Tox5-2 and a loop-mediated isothermal amplification (LAMP) procedure were used to
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Detection of Fusarium graminearum DNA using a loop-mediated
2010.6.15 During the current study a LAMP assay was developed for the specific detection of Fusarium graminearum, the major causative agent of Fusarium head
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qPCR and loop mediated isothermal amplification for rapid
2019.9.30 An optimized protocol for DNA extraction from wheat seeds and loop-mediated isothermal amplification (LAMP) to detect Fusarium graminearum
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An optimized protocol for DNA extraction from wheat seeds and
Specific PCR primer pair Tox5-1/Tox5-2 and a loop-mediated isothermal amplification (LAMP) procedure were used to detect genomic DNA of Fusarium graminearum in
Charlar en Línea
Detection of Fusarium graminearum DNA using a loop-mediated
2010.6.15 Results obtained indicate that LAMP offers an interesting new assay format for the rapid and specific DNA-based detection and identification of agriculturally
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The application of loop-mediated isothermal amplification (LAMP)
2013.12.1 An optimized protocol for DNA extraction from wheat seeds and loop-mediated isothermal ampliﬁcation (LAMP) to detect Fusarium graminearum
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Development and application of loop-mediated isothermal
2014.11.18 LAMP detection of the F167Y mutation in F. graminearum and digestion of positive LAMP products. (A) LAMP for detection of the F167Y mutation using HNB as
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Detection of Fusarium graminearum DNA using a loop-mediated
2010.6.15 We describe the development and application of a LAMP-based assay for the detection and identification of F. graminearum in pure cultures and in contaminated
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Loop-Mediated Isothermal Amplification-Based Detection of
2012.1.1 Here we describe the use of loop-mediated isothermal amplification (LAMP) with indirect in-tube detection of DNA amplification as a rapid and robust method.
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An optimized protocol for DNA extraction from wheat seeds and
2011.6.1 An optimized protocol for DNA extraction from wheat seeds and Loop-Mediated Isothermal Amplification (LAMP) to detect Fusarium graminearum contamination of wheat grain. Sign in Create an account. orcid. Europe PMC. Menu. About. About Europe PMC; Preprints in Europe PMC; Funders; Joining Europe
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Detecting Fusarium oxysporum f. sp. elaeidis by using loop
2023.9.25 We developed a Loop-Mediated Isothermal Amplification (LAMP) to detect Fusarium oxysporum f. sp. elaeidis (FOE) causal agent of fusarium wilt in oil palm. The assay was designed based on partial sequences of effector protein Secreted In Xylem (SIX8, SIX10 and SIX13) and P450 cytochrome-oxidase. The isolates used were
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DETECTION OF FUSARIUM OXYSPORUM F. SP.
2019.2.4 184 Wheat Seeds and Loop-Mediated Isothermal Amplification (LAMP) to Detect Fusarium graminearum 185 Contamination of Wheat Grain. International Journal of Molecular
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Evaluation of loop-mediated isothermal amplification (LAMP)
2008.2.1 Specific PCR primer pair Tox5-1/Tox5-2 and a loop-mediated isothermal amplification (LAMP) procedure were used to detect genomic DNA of Fusarium graminearum in contaminated wheat seeds.
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Semantic Scholar - [PDF] One-hour loop-mediated isothermal ...
This assay will support plant quarantine programs in Saudi Arabia and Gulf Cooperation Council states, to prevent the introduction of foreign FHB species, and detect genomic DNA of F. graminearum in fungal pure culture and in contaminated feed samples. Fusarium head blight (FHB) caused by several Fusarium species is one of the most serious
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qPCR and loop mediated isothermal amplification for rapid detection
2019.5.22 Primarily, the specificity of the primer was assessed by using genomic DNA of U. tritici, Fusarium graminearum, Blumeria graminis, Rhizoctonia cerealis, Puccinia striiformis, Bipolaris sorokiniana, and Alternaria solani as templates. Further, the amplification systems were optimized. Finally, the sensitivity of qPCR and LAMP assays
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Molecular detection of Fusarium graminearum causing head
2020.7.8 A rapid and efficient loop-mediated isothermal amplification (LAMP) method was developed for the detection of Fusarium graminearum, predominant Fusarium spp. for causing head blight of wheat in many wheat growing countries including India. The four LAMP primers were designed from partial sequence of Fusarium
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Detection of Fusarium oxysporum f. sp. fragariae by Using Loop
2021.2.23 We developed a loop-mediated isothermal amplification (LAMP) assay for detecting Fusarium oxysporum f. sp. fragariae, the causal agent of wilt in strawberry plants. This assay was based on genomic regions between the portions of transposable elements Han and Skippy of the fungus. The LAMP assay allowed the efficient detection of F.
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IJMS Free Full-Text An Optimized Protocol for DNA Extraction
2011.6.1 Specific PCR primer pair Tox5-1/Tox5-2 and a loop-mediated isothermal ampliﬁcation (LAMP) procedure were used to detect genomic DNA of Fusarium graminearum in contaminated wheat seeds. In this method there is no need to use liquid nitrogen for crushing germinated seedlings.
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[PDF] An Optimized Protocol for DNA Extraction from Wheat
2011.6.1 This method is a fast and cost-effective alternative to traditional diagnostic methods for the early detection of toxigenic fusaria in cereals and using a loop-mediated isothermal amplification procedure was used to detect genomic DNA of Fusarium graminearum in contaminated wheat seeds. A simple, rapid, and efficient method for
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qPCR and loop mediated isothermal amplification for rapid
2019.9.30 An optimized protocol for DNA extraction from wheat seeds and loop-mediated isothermal amplification (LAMP) to detect Fusarium graminearum contamination of wheat grain. International Journal of Molecular Sciences. 2011; 12 (6):3459–3472. doi: 10.3390/ijms12063459. [PMC free article] [Google Scholar]
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Agarose gel electrophoresis of LAMP reactions with a
Niessen et al. [118] developed a GAOA gene (galactose oxidase)-based LAMP assay for the detection of Fusarium graminearum, a species that produces different mycotoxins and causes Fusarium head ...
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Loop-Mediated Isothermal Amplification-Based Detection of Fusarium ...
2013.1.8 To overcome these problems, a loop-mediated isothermal amplification (LAMP) assay was developed to detect F. graminearum. This assay works at 65 • C and can detect F. graminearum within 30-60 ...
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(PDF) qPCR and loop mediated isothermal amplification for rapid ...
2019.9.30 we used qPCR and LAMP assays to rapidly detect wheat loose smut (Kuboki et al., 2003; Poon et al., 2005; Kono et al., 2004). ... detect Fusarium graminearum contamination of wheat grain.
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Detection of Fusarium oxysporum f. sp. fragariae by Using Loop
2021.2.23 We developed a loop-mediated isothermal amplification (LAMP) assay for detecting Fusarium oxysporum f. sp. fragariae, the causal agent of wilt in strawberry plants. This assay was based on genomic regions between the portions of transposable elements Han and Skippy of the fungus. The LAMP assay allowed the efficient detection of F.
Charlar en Línea
An optimized protocol for DNA extraction from wheat seeds and
2011.6.1 An optimized protocol for DNA extraction from wheat seeds and Loop-Mediated Isothermal Amplification (LAMP) to detect Fusarium graminearum contamination of wheat grain. Sign in Create an account. orcid. Europe PMC. Menu. About. About Europe PMC; Preprints in Europe PMC; Funders; Joining Europe
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The application of loop-mediated isothermal amplification (LAMP)
2013.12.1 While many of the mycotoxigenic Fusarium species are destructive plant pathogens producing mycotoxins before, or immediately after commodities were. Future prospects of LAMP in food safety testing. The greatest potential of LAMP lies in its sensitivity, rapidness and specificity, as well as convenience and efficiency in practical
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An optimized protocol for DNA extraction from wheat seeds and
2011.6.1 An optimized protocol for DNA extraction from wheat seeds and Loop-Mediated Isothermal Amplification (LAMP) to detect Fusarium graminearum contamination of wheat grain. Sign in Create an account. orcid. Europe PMC. Menu. About. About Europe PMC; Preprints in Europe PMC; Funders; Become a funder;
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Detection of Fusarium graminearum DNA using a loop-mediated
2010.6.15 To overcome the above-mentioned drawbacks, a LAMP assay was developed to detect F. graminearum (Niessen and Vogel, 2010). The LAMP product was indirectly detected using calcein fluorescence as a visualization marker without the necessity of time-consuming electrophoretic analysis (Niessen and Vogel, 2010).
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Fusarium Head Blight on Wheat: Biology, Modern Detection and
2023.3.3 Fusarium head blight (FHB) is a major threat for wheat production worldwide. Most reviews focus on Fusarium graminearum as a main causal agent of FHB. However, different Fusarium species are involved in this disease complex. These species differ in their geographic adaptation and mycotoxin profile. The incidence of FHB
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